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KMID : 1094720070120060713
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 6 p.713 ~ p.719
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Production and physicochemical characterization of β-glucan produced by Paenibacillus polymyxa JB115
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Jung Hee-Kyoung
Hong Joo-Heon
Park Seung-Chun
Park Byung-Kwon
Nam Doo-Hyun
Kim Sang-Dal
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Abstract
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This study was conducted to develop a bacterial glucan as an animal feed additive. A novel glucan-producing bacterium.Paenibacillus polymyxa JB115, was isolated from Korean soil. The glucan, JB115-BG, produced byP. polymyxa JB115, was confirmed by TLC to be composed of glucose only. By examining FT-IR,1H NMR, and13C NMR spectra, it was proven that JB115-BG has a β-(1→3)- and β-(1→6)-linked glucan structure. The particle size of JB115-BG was distributed in the range of 4?800 μm, with a mean value of 149.1 μm, and its molecular distribution ranged from 6.9∼3,103.7 kDa. It was also observed that 80% of the purified JB115-BG had a molecular distribution above 100 kDa. The obtained results suggest that the glucan JB115-BG can be used as an animal feed additive for the purpose of enhancing immunity.
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KEYWORD
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β-D-glucan, exopolysaccharide, polysaccharide structure, Paenibacillus polymyxa, feed additive
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